How do you calculate ABTS?
Percent inhibition of absorbance at 734 nm was calculated using the formula, ABTS·+ scavenging effect (%) = ((AB–AA)/ AB)×100 (2), where, AB is absorbance of ABTS radical + methanol; AA is absorbance of ABTS radical + sample extract/standard.
What is ABTS method?
The ABTS assay measures the relative ability of antioxidants to scavenge the ABTS generated in aqueous phase, as compared with a Trolox (water soluble vitamin E analogue) standard. The ABTS is generated by reacting with a strong oxidizing agent (eg, potassium permanganate or potassium persulfate) with the ABTS salt.
What is the full form of ABTS?
In biochemistry, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other.
What is the difference between ABTS and DPPH?
In specification, the ABTS assay is based on the generation of a blue/green ABTS·+ that can be reduced by antioxidants; whereas the DPPH assay is based on the reduction of the purple DPPH· to 1,1-diphenyl-2-picryl hydrazine.
How do I make 7mm ABTS?
The ABTS solution was prepared by mixing an equal volume of a 7 mmol/L ABTS stock solution with a 2.45 mmol/L potassium persulfate solution. The mixture was then stored in the dark at room temperature for 12–16 h.
How do you make a FRAP solution?
The FRAP reagent was prepared by mixing 25 ml of 300.0 mmol/L acetate buffer, 2.5 ml of 10 mmol/L TPTZ solution, and 2.5 ml of 20 mmol/L FeCl3 solution in a 10:1:1 ratio. 10 μL of sample was mixed with 200 μL of FRAP reagent; the contents were mixed vigorously.
What is ABTS substrate?
ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2′-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase.
What is FRAP test?
Ferric reducing ability of plasma (FRAP, also Ferric ion reducing antioxidant power) is an antioxidant capacity assay that uses Trolox as a standard. This assay is often used to measure the antioxidant capacity of foods, beverages and nutritional supplements containing polyphenols.
What does DPPH stand for?
DPPH is a common abbreviation for the organic chemical compound 2,2-diphenyl-1-picrylhydrazyl. It is a dark-colored crystalline powder composed of stable free radical molecules.
How do you calculate antioxidant activity by DPPH?
Lower absorbance of the reaction mixture indicated higher free radical activity. The percentage of DPPH scavenging effect was calculated by following equation. DPPH scavenging effect (%)/% Inhibition=A0-A1/A0 × 100 Where A0=The absorbance of control. A1=The absorbance of standard.
What is FRAP reagent?
What is the standard for FRAP assay?
Standards. Ascorbic acid was used as a standard for DPPH-free radical scavenging assay and for reducing power assay. Trolox and ascorbic acid served as two standards for ABTS scavenging assay and ferrus sulphate for FRAP assay. For total phenol content calculation, gallic acid was used as the standard.
What is the method of oxidation of Abts?
Oxidation of ABTS by hydrogen peroxide catalyzed by horseradish peroxidase encapsulated into sol–gel glass.: Effects of glass matrix on reactivity – ScienceDirect
What is the mechanism of oxidation of ABTS in glass matrix?
Oxidation of ABTS by hydrogen peroxide catalyzed by horseradish peroxidase encapsulated into sol–gel glass.: Effects of glass matrix on reactivity
Can peroxidase-catalyzed oxidation of Abts lead to azodication?
We summarize the findings here. In the presence of excess of H 2 O 2, peroxidase-catalyzed oxidation of ABTS can lead not only to ABTS +, but also further to azodication ABTS 2+ [13]. The latter is unstable in aqueous solution [24], [27], [28]; its decomposition products are pale-yellow or colorless.
What does Abts mean in chemistry?
The 2,2′-azinobis- (3-ethylenebenzothiazoline)-6-sulfonic acid (ABTS) assay is another commonly applied antioxidant assay. When an antioxidant is added, the ABTS loses its blue-green color and it is reduced to ABTS [139].